etc/email_templates.xml
<?xml version="1.0"?>
<config xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:noNamespaceSchemaLocation="../../Email/etc/email_templates.xsd">
<template id="statusinfo_guest" label="Email Form" file="statusinfo_guest.html" type="html" module="Vendor_Module" area="frontend"/>
</config>
write this code in
view/frontend/email/statusinfo_guest.html
Note: remember file type is "html"
{{template config_path="design/email/header_template"}}
{{trans "%order_status" order_status=$order.getFrontendStatusLabel() |raw}}
{{layout handle="email_sample_layout" order=$order area="frontend"}}
{{template config_path="design/email/footer_template"}}
now add layout file "email_sample_layout.xml" in view/frontend/layout and add below code in it
<?xml version="1.0"?>
<page xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:noNamespaceSchemaLocation="urn:magento:framework:View/Layout/etc/page_configuration.xsd" label="Email Product List" design_abstraction="custom">
<body>
<block class="Magento\Framework\View\Element\Template" name="status_info" template="Magento_Sales::email/order_update/statusinfo_guest.phtml"/>
</body>
</page>
Now write what you want to show in your phtml file "statusinfo_guest.phtml"
Hope it works.