I'm not able to add multiple items in a single wishlist, if I add a new item on same wishlist, it is replaced by previous item.
Magento EE 188.8.131.52 , Please help me to solve this issue, to my knowledge this appears to be a bug?
This was a known issue for EE1.14. Magento has fixed this in 1.14.1. You can find this in the release notes here - http://www.magentocommerce.com/knowledge-base/entry/ee114-later-release-notes (just search for 'Resolved the following issues with the wish list:' on that page).